3,4-二硝基苯基-N-乙酰-β-D-氨基葡萄糖苷，一种用于直接分光光度法测定N-乙酰-β-D-氨基葡萄糖苷酶或N-乙酰-β-D-己糖胺酶的合成底物。
3,4-Dinitrophenyl N-acetyl-beta-D-glucosaminide, a synthetic substrate for direct spectrophotometric assay of N-acetyl-beta-D-glucosaminidase or N-acetyl-beta-D-hexosaminidase.
作者信息
Yagi T, Hisada R, Shibata H
机构信息
Department of Chemistry and Chemical Education, Shizuoka University, Japan.
出版信息
Anal Biochem. 1989 Dec;183(2):245-9. doi: 10.1016/0003-2697(89)90474-0.
3,4-Dinitrophenyl N-acetyl-beta-D-glucosaminide (3,4-dnpGlcNAc) was synthesized and proposed as an artificial substrate for N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) and N-acetyl-beta-D-hexosaminidase (EC 3.2.1.52) (collectively abbreviated as NAGase). 3,4-dnpGlcNAc is water soluble and is fairly stable in aqueous medium. 3,4-Dinitrophenol released from 3,4-dnpGlcNAc by NAGase absorbs light of 400 nm at pH near 5, where the activity of urinary NAGase is assayed for diagnostic use. Direct and sensitive spectrophotometric assay of NAGase is, thus, possible using 3,4-dnpGlcNAc as an artificial substrate by monitoring the increase of A400 due to the release of 3,4-dinitrophenol in a thermostated spectrophotometer.
合成了3,4 - 二硝基苯基 - N - 乙酰 - β - D - 氨基葡萄糖苷（3,4 - dnpGlcNAc），并将其作为N - 乙酰 - β - D - 氨基葡萄糖苷酶（EC 3.2.1.30）和N - 乙酰 - β - D - 己糖胺酶（EC 3.2.1.52）（统称为NAGase）的人工底物。3,4 - dnpGlcNAc可溶于水，在水性介质中相当稳定。NAGase从3,4 - dnpGlcNAc释放出的3,4 - 二硝基苯酚在pH接近5时吸收400 nm的光，在此pH下可测定尿NAGase活性用于诊断。因此，通过在恒温分光光度计中监测由于3,4 - 二硝基苯酚释放导致的A400增加，使用3,4 - dnpGlcNAc作为人工底物对NAGase进行直接且灵敏的分光光度测定是可行的。
Zotero 插件