Sasamoto K, Ohkura Y
Dojindo Laboratories, Kumamoto, Japan.
Chem Pharm Bull (Tokyo). 1990 May;38(5):1323-5. doi: 10.1248/cpb.38.1323.
A new type of chemiluminogenic substrate for N-acetyl-beta-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide, was prepared by incorporating an enzyme-removable substituent, N-acetyl-D-glucosaminide group, into the hydrazide moiety of luminol. This substrate releases luminol upon enzymatic hydrolysis. The enzyme was detected chemiluminescently using this substrate.
通过将可被酶去除的取代基N-乙酰-D-氨基葡萄糖苷基团引入鲁米诺的酰肼部分，制备了一种新型的N-乙酰-β-D-氨基葡萄糖苷酶化学发光底物——邻氨基苯二甲酰肼基-N-乙酰-β-D-氨基葡萄糖苷。该底物在酶促水解时释放鲁米诺。使用该底物通过化学发光法检测该酶。
