Makise J, Saito E, Obuchi M, Kanayama M, Ichikawa K, Harakawa K, Yoshida K
Central Clinical Laboratory, Yokosuka Kyosai Hospital, Japan.
Clin Chem. 1988 Oct;34(10):2140-3.
In this new procedure for determining the activity of N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30; NAG) in urine, a new synthetic substrate, 2-chloro-4-nitrophenyl-N-acetyl-beta-D-glucosaminide is used. No sample blank is required because the activity is determined kinetically. Very high precision is possible, with CVs of less than 3.8% both within-run and between-run. Glucose, ascorbic acid, bilirubin, bromosulfonphthalein, and phenosulfonphthalein do not interfere. The reference interval (mean +/- 2 SD) for healthy subjects was 1.9-11.1 U per gram of creatinine. NAG activity can be determined over a wide range (2-500 U/L) with high sensitivity. The method is suitable for use with various automated analyzers, and it should be useful for screening for renal diseases.
在这种测定尿液中N-乙酰-β-D-氨基葡萄糖苷酶（EC 3.2.1.30；NAG）活性的新方法中，使用了一种新的合成底物2-氯-4-硝基苯基-N-乙酰-β-D-氨基葡萄糖苷。由于活性是通过动力学方法测定的，因此不需要样品空白。可以实现非常高的精密度，批内和批间的变异系数均小于3.8%。葡萄糖、抗坏血酸、胆红素、溴磺酚酞和酚磺酞均无干扰。健康受试者的参考区间（均值±2标准差）为每克肌酐1.9 - 11.1 U。NAG活性可以在很宽的范围内（2 - 500 U/L）进行高灵敏度测定。该方法适用于各种自动分析仪，对肾脏疾病的筛查应该是有用的。
