Motoyoshi H, Tanaka S, Nagata Y, Yamasaki H, Fukuda K, Kita H
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka.
Nihon Seikeigeka Gakkai Zasshi. 1989 Dec;63(12):1464-8.
Using the monoclonal antibody to the keratan sulfate(KS), we modified the assay system for detecting of serum KS levels (Thonar, et al. Arthritis Rheum. 28:1367-1376, 1985). This method is based on the enzyme-linked immunosorbent assay (ELISA). The dogfish KS was most effective and sensitive, in this system. KS levels were measured in 45 patients with no complaints of arthralgia, no history of arthritis and no remarkable change in the laboratory data, including erythrocyte sedimentation rates, rheumatoid factor, uric acid and C-reactive protein. Age related changes were apparent in serum levels of KS. Age related catabolism in normal articular cartilage and serum KS levels will aid to estimate the turnover of articular proteoglycan.
我们使用抗硫酸角质素（KS）单克隆抗体，改良了检测血清KS水平的分析系统（托纳尔等人，《关节炎与风湿病》，28:1367 - 1376，1985年）。该方法基于酶联免疫吸附测定法（ELISA）。在这个系统中，鲨鱼硫酸角质素最为有效且灵敏。对45例无关节疼痛主诉、无关节炎病史且实验室数据（包括红细胞沉降率、类风湿因子、尿酸和C反应蛋白）无显著变化的患者进行了KS水平测定。血清KS水平存在明显的年龄相关变化。正常关节软骨的年龄相关分解代谢以及血清KS水平将有助于评估关节蛋白聚糖的更新情况。
