Akdere H, Tastekin E, Mericliler M, Burgazli K M
Department of Urology, Faculty of Medicine, University of Trakya, Edirne, Turkey.
Eur Rev Med Pharmacol Sci. 2014 Oct;18(19):2936-41.
The aim of the study was to investigate the protective effects of Ginkgo biloba EGb761 extract on renal ischemia-reperfusion (I/R) injury in rats.
26 male Wistar albino rats were divided into four groups: First group (n=6), which served as control received only standard pellet; second group (IR) (n=6) was subjected to renal I/R injury; a third group (Gb) (n=7) was given additional EGb761 extract; and rats in the fourth group (IR-Gb) (n=7) had been treated with EGb761 extract before they were subjected to I/R injury. After rats were euthanized, renal tissues were analyzed microscopically, and tissue malondyaldehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) levels were determined.
MDA values were significantly high in the IR group when compared with the other groups. No significant difference in MDA values between the Control and Gb groups was observed. SOD enzyme activity was significantly lower in the IR group when compared with other groups. Furthermore, SOD values were found to be comparable in control, Gb and IR-Gb groups. The CAT enzymatic activity was significantly low in the IR group when compared with the other groups. Moreover, although no statistical significance was identified between control group and Gb group, CAT levels in these groups were higher compared to IR-Gb group. Microscopic examination showed no histopathological differences between the control and Gb groups. Cast formation and tubular necrosis in the IR group have been determined to be significantly high when compared with IR-Gb group. We further observed that the histopathological changes in the IR-Gb group were lesser in the advanced levels when compared with the IR group.
Ginkgo biloba Egb761 extract applied before renal ischemia-reperfusion decreases the tissue damage.
本研究旨在探讨银杏叶提取物EGb761对大鼠肾缺血再灌注（I/R）损伤的保护作用。
将26只雄性Wistar白化大鼠分为四组：第一组（n = 6）作为对照组，仅给予标准颗粒饲料；第二组（IR）（n = 6）进行肾I/R损伤；第三组（Gb）（n = 7）额外给予EGb761提取物；第四组（IR-Gb）（n = 7）在进行I/R损伤前用EGb761提取物处理。大鼠安乐死后，对肾组织进行显微镜分析，并测定组织丙二醛（MDA）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平。
与其他组相比，IR组的MDA值显著升高。对照组和Gb组的MDA值未观察到显著差异。与其他组相比，IR组的SOD酶活性显著降低。此外，对照组、Gb组和IR-Gb组的SOD值相当。与其他组相比，IR组的CAT酶活性显著降低。此外，虽然对照组和Gb组之间未发现统计学差异，但这两组的CAT水平高于IR-Gb组。显微镜检查显示对照组和Gb组之间无组织病理学差异。与IR-Gb组相比，IR组的管型形成和肾小管坏死显著增加。我们进一步观察到，与IR组相比，IR-Gb组晚期的组织病理学变化较小。
在肾缺血再灌注前应用银杏叶提取物Egb761可减少组织损伤。
