Neurophysiology Laboratory, Neurosurgical Service, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Physiol Rep. 2024 Jun;12(11):e16050. doi: 10.14814/phy2.16050.
In posterior spine surgery, retractors exert pressure on paraspinal muscles, elevating intramuscular pressure and compromising blood flow, potentially causing muscle injury during ischemia-reperfusion. Ginkgo biloba extract (EGb 761), known for its antioxidant and free radical scavenging properties and its role in treating cerebrovascular diseases, is investigated for its protective effects against muscle ischemia-reperfusion injury in vitro and in vivo. Animals were randomly divided into the control group, receiving normal saline, and experimental groups, receiving varying doses of EGb761 (25/50/100/200 mg/kg). A 2-h hind limb tourniquet-induced ischemia was followed by reperfusion. Blood samples collected pre-ischemia and 24 h post-reperfusion, along with muscle tissue samples after 24 h, demonstrated that EGb761 at 1000 μg/mL effectively inhibited IL-6 and TNF-α secretion in RAW 264.7 cells without cytotoxicity. EGb761 significantly reduced nitric oxide (NO) and malondialdehyde (MDA) levels, myeloperoxidase (MPO) activity, and increased glutathione (GSH) levels compared to the control after 24 h. Muscle tissue sections revealed more severe damage in the control group, indicating EGb761's potential in mitigating inflammatory responses and oxidative stress during ischemia-reperfusion injury, effectively protecting against muscle damage.
在后脊柱手术中，牵开器对椎旁肌肉施加压力，导致肌肉内压升高，血液流动受限，在缺血再灌注过程中可能导致肌肉损伤。银杏叶提取物 (EGb 761) 以其抗氧化和清除自由基的特性以及在治疗脑血管疾病方面的作用而闻名，其在体外和体内对肌肉缺血再灌注损伤的保护作用已被研究。动物随机分为对照组，给予生理盐水；实验组，给予不同剂量的 EGb761（25/50/100/200mg/kg）。采用 2 小时后肢止血带诱导缺血，然后再灌注。缺血前和再灌注后 24 小时采集血样，并在 24 小时后采集肌肉组织样本，结果表明，1000μg/mL 的 EGb761 在没有细胞毒性的情况下，可有效抑制 RAW 264.7 细胞中 IL-6 和 TNF-α 的分泌。与对照组相比，EGb761 可显著降低 NO 和 MDA 水平、髓过氧化物酶 (MPO) 活性，并增加 GSH 水平。24 小时后，肌肉组织切片显示对照组损伤更严重，表明 EGb761 具有减轻缺血再灌注损伤过程中的炎症反应和氧化应激的潜力，能有效保护肌肉免受损伤。
