Huang Hui, Wang Xia, Yang Ling, He Wenxiang, Meng Tiantian, Zheng Ke, Xia Xin, Zhou Yingjun, He Jianhua, Liu Chunming, Zou Shengwen, Xiao Dingfu
College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Yiyang Vocational and Technical College, Yiyang, China.
Front Vet Sci. 2022 Jun 23;9:882754. doi: 10.3389/fvets.2022.882754. eCollection 2022.
In order to study the regulation of Fenugreek seed extract (FSE) on the immunity of broilers, and explore the appropriate amount of FSE in broilers' production, 1-day-old yellow feather broilers with a total of 420 birds were randomly allocated into seven treatments. Each treatment had six replicates, with 10 birds per replicate. The two control groups were the basic fodder group fed with basal diet and the bacitracin zinc group added 30 mg/kg bacitracin zinc to the basal diet. Experimental groups included five levels of FSE (50, 100, 200, 400, and 800 mg/kg FSE to the basal diet, respectively). The pre-test period was 7 days and the formal test lasted for 56 days. The results showed that the average daily gain (ADG) of 50 and 800 mg/kg FSE groups was significantly increased ( < 0.01), and the feed to gain ratio (F/G) of FSE groups was significantly decreased ( < 0.01) compared with the basic fodder and the bacitracin zinc groups. Compared with the basic fodder group, the serum total cholesterol (TC) content in the FSE groups was significantly decreased ( < 0.05), the serum low density lipoprotein cholesterol (LDL-C) content of 50, 100, and 800 mg/kg FSE groups was significantly lower than that of the basic fodder group ( < 0.05). Compared with the basic fodder and bacitracin zinc groups, the serum immunoglobulins (IgG, IgM, IgA) content of 100 and 200 mg/kg FSE groups were significantly increased ( < 0.05). Compared with the bacitracin zinc group, the serum interleukins (IL-1, IL-10) content of 400 mg/kg FSE group were significantly increased ( ≤ 0.05), and the serum interferon-γ (IFN-γ) content of 100 and 200 mg/kg FSE groups was significantly increased ( < 0.05). Compared with the basic fodder group, the lower doses (0-400 mg/kg) of FSE had no significant effect on the mRNA expression of toll-like receptors 4/ myeloid differentiation factor 88/ nuclear factor-κB (TLR4/MyD88/NF-κB) signaling pathways ( > 0.05). The 800 mg/kg FSE treatment group significantly increased the expression levels of nuclear factor-κB (NF-κB) mRNA in the spleen of broilers ( < 0.05). The zinc bacitracin group significantly increased the expression levels of myeloid differentiation factor 88 (MyD88) and nuclear factor-κB (NF-κB) mRNA ( ≤ 0.05). The results showed that FSE could promote the secretion of immunoglobulins, regulate the body's cytokines, and have a positive effect on immunity in broilers. Furthermore, the recommended supplement of FSE is 100 mg/kg in the broiler diet.
为研究胡芦巴籽提取物（FSE）对肉鸡免疫力的调节作用，探索其在肉鸡生产中的适宜添加量，将420只1日龄黄羽肉鸡随机分为7个处理组。每个处理组设6个重复，每个重复10只鸡。两个对照组分别为饲喂基础日粮的基础饲料组和在基础日粮中添加30 mg/kg杆菌肽锌的杆菌肽锌组。试验组包括5个FSE添加水平（分别在基础日粮中添加50、100、200、400和800 mg/kg FSE）。预试期7天，正式试验期56天。结果表明，与基础饲料组和杆菌肽锌组相比，50和800 mg/kg FSE组的平均日增重（ADG）显著提高（P<0.01），FSE各处理组的料重比（F/G）显著降低（P<0.01）。与基础饲料组相比，FSE各处理组血清总胆固醇（TC）含量显著降低（P<0.05），50、100和800 mg/kg FSE组血清低密度脂蛋白胆固醇（LDL-C）含量显著低于基础饲料组（P<0.05）。与基础饲料组和杆菌肽锌组相比，100和200 mg/kg FSE组血清免疫球蛋白（IgG、IgM、IgA）含量显著升高（P<0.05）。与杆菌肽锌组相比，400 mg/kg FSE组血清白细胞介素（IL-1、IL-10）含量显著升高（P≤0.05），100和200 mg/kg FSE组血清干扰素-γ（IFN-γ）含量显著升高（P<0.05）。与基础饲料组相比，较低剂量（0～400 mg/kg）的FSE对肉鸡Toll样受体4/髓样分化因子88/核因子κB（TLR4/MyD88/NF-κB）信号通路mRNA表达无显著影响（P>0.05）。800 mg/kg FSE处理组显著提高了肉鸡脾脏核因子κB（NF-κB）mRNA表达水平（P<0.05）。杆菌肽锌组显著提高了髓样分化因子88（MyD88）和核因子κB（NF-κB）mRNA表达水平（P≤0.05）。结果表明，FSE能促进免疫球蛋白分泌，调节机体细胞因子，对肉鸡免疫力有积极作用。此外，推荐在肉鸡日粮中添加FSE的量为100 mg/kg。
