Anuradha C V, Ravikumar P
Department of Biochemistry, Annamalai University, Annamalainagar-608 002.
Indian J Physiol Pharmacol. 2001 Oct;45(4):408-20.
The influence of fenugreek seed powder supplementation in the diet on lipid peroxidation and antioxidant status was studied in normal and alloxan-diabetic rats. The protective effect of the aqueous extract of the seeds on the activity of calcium-dependent adenosinetriphosphatase (Ca2+ ATPase) in liver homogenate in the presence of Fe2+/ascorbate in vitro was also investigated. Normal and diabetic rats were provided with a diet supplemented with fenugreek seed powder for 30 days at a dosage of 2 g/kg body weight. The diabetic rats exhibited enhanced lipid peroxidation and increased susceptibility to oxidative stress associated with depletion of antioxidants in liver, kidney and pancreas. However, treatment with fenugreek seed powder normalised the alterations. In normal rats supplementation resulted in increased antioxidant status with reduction in peroxidation. Ca2+ ATPase activity in liver was protected by the aqueous extract to nearly 80% of the initial activity. The findings suggest that the soluble portion of the seeds could be responsible for the antioxidant property.
研究了在正常和四氧嘧啶诱导的糖尿病大鼠饮食中添加胡芦巴籽粉对脂质过氧化和抗氧化状态的影响。还研究了种子水提取物在体外Fe2+/抗坏血酸存在下对肝匀浆中钙依赖性三磷酸腺苷酶（Ca2+ATPase）活性的保护作用。正常和糖尿病大鼠以2 g/kg体重的剂量喂食添加了胡芦巴籽粉的饮食30天。糖尿病大鼠表现出脂质过氧化增强，以及与肝脏、肾脏和胰腺中抗氧化剂消耗相关的氧化应激易感性增加。然而，用胡芦巴籽粉治疗可使这些改变恢复正常。在正常大鼠中，添加物导致抗氧化状态增加，过氧化减少。肝脏中的Ca2+ATPase活性受到水提取物的保护，接近初始活性的80%。研究结果表明，种子的可溶部分可能是抗氧化特性的原因。
