van der Geest Kornelis S M, Smigielska-Czepiel Katarzyna, Park Ji-Ah, Abdulahad Wayel H, Kim Hye-Won, Kroesen Bart-Jan, van den Berg Anke, Boots Annemieke M H, Lee Eun-Bong, Brouwer Elisabeth
Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Department of Pathology and Medical Biology and Department of Laboratory Medicine, Section Medical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Department of Pathology and Medical Biology and Department of Laboratory Medicine, Section Medical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands
Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, Division of Rheumatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea, Department of Pathology and Medical Biology and Department of Laboratory Medicine, Section Medical Immunology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Rheumatology (Oxford). 2015 May;54(5):950-8. doi: 10.1093/rheumatology/keu407. Epub 2014 Oct 21.
The aim of this study was to investigate the turnover of Treg cells in the SF of RA patients.
Treg cells were enumerated in peripheral blood and SF of RA patients and analysed by flow cytometry for expression of the proliferation marker Ki-67 and binding of the apoptosis marker annexin V. Sorted Treg cells of RA patients were analysed for expression of anti-apoptotic regulators Bcl-2 and microRNA-21 (miR-21) by RT-PCR.
Treg cells displaying a memory phenotype were abundant in the SF of RA patients. SF Treg cells more frequently expressed the proliferation marker Ki-67 than conventional T cells. Only few SF Treg cells were apoptotic, as indicated by limited annexin V staining of these cells. SF Treg cells displayed high transcription levels of Bcl-2 and miR-21 in comparison with SF conventional T cells and peripheral blood Treg cells.
Treg cells with a memory phenotype accumulate in the SF of RA patients. These Treg cells have a high proliferative activity and demonstrate little apoptosis. The latter finding could be explained by high transcription of Bcl-2 and miR-21 in SF Treg cells.
本研究旨在调查类风湿关节炎（RA）患者滑液（SF）中调节性T细胞（Treg细胞）的周转率。
对RA患者外周血和滑液中的Treg细胞进行计数，并通过流式细胞术分析增殖标志物Ki-67的表达以及凋亡标志物膜联蛋白V的结合情况。通过逆转录聚合酶链反应（RT-PCR）分析RA患者分选的Treg细胞中抗凋亡调节因子Bcl-2和微小RNA-21（miR-21）的表达。
具有记忆表型的Treg细胞在RA患者的滑液中大量存在。与传统T细胞相比，滑液Treg细胞更频繁地表达增殖标志物Ki-67。这些细胞的膜联蛋白V染色有限，表明只有少数滑液Treg细胞发生凋亡。与滑液传统T细胞和外周血Treg细胞相比，滑液Treg细胞显示出Bcl-2和miR-21的高转录水平。
具有记忆表型的Treg细胞在RA患者的滑液中积聚。这些Treg细胞具有高增殖活性，且很少发生凋亡。后一发现可通过滑液Treg细胞中Bcl-2和miR-21的高转录来解释。
