Pelikánová T, Kohout M, Hilgertová J, Base J, Farská I, Válek J
Institute for Clinical and Experimental Medicine, Faculty of General Medicine, Charles University, Prague.
Physiol Bohemoslov. 1989;38(5):419-25.
The cell membrane plays an important role in the mechanism of insulin action. To test whether erythrocyte insulin receptor characteristics are related to the erythrocyte membrane lipid composition, 11 healthy volunteers were studied. The relationship between insulin binding to erythrocytes, the number of receptors per cell and the affinity of receptors to insulin on the one hand and total phospholipid fatty acid (FA) composition and cholesterol/phospholipid molar ratio in the erythrocyte membrane on the other hand were evaluated. 1. We found a significant negative correlation between specific insulin binding and the proportion of n-6 essential FA in erythrocyte membrane phospholipids, especially linoleic acid (r = -0.82, p less than 0.01) and arachidonic acid (r = -0.73, p less than 0.05). On the other hand, a significant positive correlation between insulin binding and the proportion of nonessential FA (r = +0.65, p less than 0.05) was seen. Number of receptors per cell and the affinity of receptors were not significantly related to phospholipid FA composition. 2. There was no significant correlation between insulin receptor characteristics and the cholesterol/phospholipid molar ratio in the erythrocyte membrane. The data presented support the hypothesis that the FA pattern of membrane total phospholipids may modify the properties of insulin receptors.
细胞膜在胰岛素作用机制中发挥着重要作用。为了测试红细胞胰岛素受体特征是否与红细胞膜脂质组成相关，对11名健康志愿者进行了研究。评估了一方面胰岛素与红细胞的结合、每个细胞的受体数量以及受体对胰岛素的亲和力，与另一方面红细胞膜中总磷脂脂肪酸（FA）组成和胆固醇/磷脂摩尔比之间的关系。1. 我们发现特异性胰岛素结合与红细胞膜磷脂中n-6必需脂肪酸的比例之间存在显著负相关，尤其是亚油酸（r = -0.82，p小于0.01）和花生四烯酸（r = -0.73，p小于0.05）。另一方面，观察到胰岛素结合与非必需脂肪酸比例之间存在显著正相关（r = +0.65，p小于0.05）。每个细胞的受体数量和受体亲和力与磷脂脂肪酸组成无显著相关性。2. 胰岛素受体特征与红细胞膜中胆固醇/磷脂摩尔比之间无显著相关性。所呈现的数据支持这样的假设，即膜总磷脂的脂肪酸模式可能会改变胰岛素受体的特性。
