Nagy Károly, Rácz Gábor, Matsumoto Takashi, Ádány Róza, Ádám Balázs
Department of Preventive Medicine, Faculty of Public Health, University of Debrecen, P.O. Box 9, Debrecen H-4012, Hungary.
Department of Preventive Medicine, Faculty of Public Health, University of Debrecen, P.O. Box 9, Debrecen H-4012, Hungary; Institute of Public Health, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Aug;770:1-5. doi: 10.1016/j.mrgentox.2014.05.001. Epub 2014 May 14.
Phenothrin, a synthetic pyrethroid compound, is widely used to control agricultural and household insects, as well as to eliminate human louse infestation. Toxicity studies on the direct DNA-damaging effect of phenothrin are lacking. We therefore investigated whether phenothrin exposure can lead to increased DNA damage in vitro in human peripheral blood lymphocytes and in human hepatocytes. Genotoxicity was evaluated by means of the comet assay modified with formamidopyrimidine DNA-glycosylase post-treatment for the detection of oxidative base-damage in DNA. We also assessed the cytotoxic potential of this compound by use of combined fluorescence viability staining. Our results show that phenothrin induces statistically significant, dose-dependent DNA damage in the absence of marked cytotoxicity at concentrations higher than 20 μM and 50 μM in human blood peripheral lymphocytes and hepatocytes, respectively. Oxidative DNA damage could also be detected in the two cell types, although this did not reach statistical significance. These findings provide evidence of the DNA-damaging potential of phenothrin and call for additional studies to reveal the genotoxic properties of this pyrethroid. The observations also point at the importance of using caution when considering the use of phenothrin.
氯菊酯是一种合成拟除虫菊酯化合物，广泛用于防治农业害虫和家庭害虫，以及消除人体虱虫感染。目前缺乏关于氯菊酯直接DNA损伤作用的毒性研究。因此，我们研究了氯菊酯暴露是否会在体外导致人外周血淋巴细胞和人肝细胞的DNA损伤增加。通过用甲酰胺嘧啶DNA糖基化酶后处理改良的彗星试验来评估遗传毒性，以检测DNA中的氧化碱基损伤。我们还通过联合荧光活力染色评估了该化合物的细胞毒性潜力。我们的结果表明，在人外周血淋巴细胞和肝细胞中，氯菊酯分别在高于20μM和50μM的浓度下诱导出具有统计学意义的剂量依赖性DNA损伤，且无明显细胞毒性。在这两种细胞类型中也能检测到氧化性DNA损伤，尽管这未达到统计学意义。这些发现提供了氯菊酯具有DNA损伤潜力的证据，并呼吁进行更多研究以揭示这种拟除虫菊酯的遗传毒性特性。这些观察结果还指出了在考虑使用氯菊酯时谨慎行事的重要性。
