a Genotoxicología Ambiental, Departamento de Ciencias Ambientales , Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México , Mexico.
b Instituto de Investigación en Ambiente y Salud , Universidad de Occidente , Los Mochis , Sinaloa , Mexico.
Toxicol Mech Methods. 2018 May;28(4):268-278. doi: 10.1080/15376516.2017.1402977. Epub 2018 Jan 18.
In the present study, human peripheral blood lymphocytes were exposed in vitro to 0, 6, 12, 18, 24, and 30 μg/mL Furia180 SC (zeta-cypermethrin) and 0, 6.3, 12.5, 18.8, 25, and 31.3 μg/mL Bulldock125 SC (β-cyfluthrin). Exposure to 32 µg/mL bleomycin for 24 h served as a positive control. The cytotoxic and genotoxic effects of each insecticide were analyzed using alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated through three genotoxicity parameters: tail length (TL), tail moment (TM) and tail intensity (TI). Furia180 SC and Bulldock125 SC pyrethroid insecticides and bleomycin significantly increased DNA damage in a concentration-dependent manner. Bulldock125 SC induced more DNA damage than Furia. Lymphocyte viability did not change after exposure to different concentrations of the two pyrethroid insecticides and bleomycin. Moreover, genotoxic results demonstrated that Furia180 SC and Bulldock125 SC insecticides caused in vitro DNA damage in human peripheral lymphocytes.
在本研究中，体外将人外周血淋巴细胞暴露于 0、6、12、18、24 和 30μg/ml Furia180 SC（Zeta-氯氰菊酯）和 0、6.3、12.5、18.8、25 和 31.3μg/ml Bulldock125 SC（β-氯氟氰菊酯）中。以 32μg/ml 博来霉素处理 24h 作为阳性对照。使用碱性彗星和台盼蓝染料排除试验分析每种杀虫剂的细胞毒性和遗传毒性作用。通过三个遗传毒性参数评估 DNA 损伤：尾部长度（TL）、尾部矩（TM）和尾部强度（TI）。Furia180 SC 和 Bulldock125 SC 拟除虫菊酯杀虫剂和博来霉素以浓度依赖性方式显著增加 DNA 损伤。Bulldock125 SC 引起的 DNA 损伤比 Furia180 SC 多。暴露于两种拟除虫菊酯杀虫剂和博来霉素的不同浓度后，淋巴细胞活力没有变化。此外，遗传毒性结果表明，Furia180 SC 和 Bulldock125 SC 杀虫剂在体外引起人外周血淋巴细胞的 DNA 损伤。
