In vitro cytotoxicity and genotoxicity of Furia180 SC (zeta-cypermethrin) and Bulldock 125SC (β-cyfluthrin) pyrethroid insecticides in human peripheral blood lymphocytes.

In the present study, human peripheral blood lymphocytes were exposed in vitro to 0, 6, 12, 18, 24, and 30 μg/mL Furia180 SC (zeta-cypermethrin) and 0, 6.3, 12.5, 18.8, 25, and 31.3 μg/mL Bulldock125 SC (β-cyfluthrin). Exposure to 32 µg/mL bleomycin for 24 h served as a positive control. The cytotoxic and genotoxic effects of each insecticide were analyzed using alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated through three genotoxicity parameters: tail length (TL), tail moment (TM) and tail intensity (TI). Furia180 SC and Bulldock125 SC pyrethroid insecticides and bleomycin significantly increased DNA damage in a concentration-dependent manner. Bulldock125 SC induced more DNA damage than Furia. Lymphocyte viability did not change after exposure to different concentrations of the two pyrethroid insecticides and bleomycin. Moreover, genotoxic results demonstrated that Furia180 SC and Bulldock125 SC insecticides caused in vitro DNA damage in human peripheral lymphocytes.