Balestrini Jenna L, Niklason Laura E
Department of Pathology, Yale University School of Medicine, New Haven, CT, 06520, USA.
Ann Biomed Eng. 2015 Mar;43(3):568-76. doi: 10.1007/s10439-014-1167-5. Epub 2014 Oct 25.
Extracellular matrix has manifold roles in tissue mechanics, guidance of cellular behavior, developmental biology, and regenerative medicine. Over the past several decades, various pre-clinical and clinical studies have shown that many connective tissues may be replaced and/or regenerated using suitable extracellular matrix scaffolds. More recently, decellularization of lung tissue has shown that gentle removal of cells can leave behind a "footprint" within the matrix that may guide cellular adhesion, differentiation and homing following cellular repopulation. Fundamental issues like understanding matrix composition and micro-mechanics remain difficult to tackle, largely because of a lack of available assays and tools for systematically characterizing intact matrix from tissues and organs. This review will critically examine the role of engineered and native extracellular matrix in tissue and lung regeneration, and provide insights into directions for future research and translation.
细胞外基质在组织力学、细胞行为引导、发育生物学和再生医学中具有多种作用。在过去几十年中，各种临床前和临床研究表明，使用合适的细胞外基质支架可以替代和/或再生许多结缔组织。最近，肺组织去细胞化研究表明，温和去除细胞后，基质中会留下一个“足迹”，这可能会在细胞重新植入后引导细胞黏附、分化和归巢。诸如理解基质组成和微观力学等基本问题仍然难以解决，这主要是因为缺乏用于系统表征组织和器官中完整基质的可用检测方法和工具。本综述将批判性地审视工程化和天然细胞外基质在组织和肺再生中的作用，并为未来研究和转化方向提供见解。
