Sicari Brian M, Zhang Li, Londono Ricardo, Badylak Stephen F
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Methods Mol Biol. 2014;1202:103-10. doi: 10.1007/7651_2013_37.
The endogenous chemotaxis of cells toward sites of tissue injury and/or biomaterial implantation is an important component of the host response. Implanted biomaterials capable of recruiting host stem/progenitor cells to a site of interest may obviate challenges associated with cell transplantation. An assay for the identification and quantification of chemotaxis induced by surgically placed biologic scaffolds composed of extracellular matrix is described herein.
细胞向组织损伤部位和/或生物材料植入部位的内源性趋化作用是宿主反应的重要组成部分。能够将宿主干细胞/祖细胞募集到感兴趣部位的植入生物材料可能会消除与细胞移植相关的挑战。本文描述了一种用于鉴定和定量由手术放置的细胞外基质组成的生物支架诱导的趋化作用的测定方法。
