一种用于植物组织的光学透明技术，可实现深度成像并与荧光显微镜兼容。
An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.
作者信息
Warner Cherish A, Biedrzycki Meredith L, Jacobs Samuel S, Wisser Randall J, Caplan Jeffrey L, Sherrier D Janine
机构信息
Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303
Delaware Biotechnology Institute (C.A.W., S.S.J., J.C., D.J.S.) andDepartments of Biological Sciences (C.A.W., M.L.B., S.S.J., J.C., D.J.S.); andPlant and Soil Sciences (M.L.B., R.J.W., J.C., D.J.S.), University of Delaware, Newark, Delaware 19717-1303.
出版信息
Plant Physiol. 2014 Dec;166(4):1684-7. doi: 10.1104/pp.114.244673. Epub 2014 Oct 24.
Abstract
We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process.
摘要
我们报道了一种无损透明化技术，该技术可增强不同植物物种标本的光传输，为基于显微镜的植物研究带来了独特机遇。透明化处理后，植物器官和厚组织切片适合进行深度成像。该透明化方法与免疫细胞化学技术兼容，可与常见荧光探针协同使用，包括广泛应用的蛋白质标签如绿色荧光蛋白（GFP），其荧光在透明化过程中得以保留。
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