Kondo Yuki, Fujita Takashi, Sugiyama Munetaka, Fukuda Hiroo
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mol Plant. 2014 Oct 25. doi: 10.1093/mp/ssu122.
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular developmen in detail. Here, we establish a novel in vitro experimental system, in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDR:GUS and TDR:YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF indicates that the key signaling TDIF- TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with the analysis using the rich Arabidopsis material resources including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation.
在维管发育过程中，原形成层细胞和形成层细胞会产生木质部和韧皮部细胞。由于维管组织深埋其中，详细分析维管发育过程一直很困难。在此，我们建立了一种新型体外实验系统，其中使用糖原合酶激酶3蛋白的抑制剂比基尼宁在拟南芥叶盘培养物中诱导维管发育。转录组分析表明，叶盘中的叶肉细胞同步转变为原形成层细胞，然后分化为管状分子。来自表达原形成层细胞标记物TDR:GUS和TDR:YFP的植物的叶盘培养物可用于原形成层细胞形成的时空可视化。用tdr突变体和TDIF进行的进一步分析表明，关键信号TDIF-TDR-GSK3s调节叶盘培养物中的木质部分化。这种新的培养系统可以与使用包括细胞标记系和突变体在内的丰富拟南芥材料资源的分析相结合，从而为分析木质部细胞分化提供了一个强大的工具。
