Sharma Arvind, Deshmukh Arunaditya, Sharma Richa, Kumar Ashwani, Mukherjee Sayantan, Chandra G C, Gakhar S K
Indian J Exp Biol. 2014 Oct;52(10):996-1002.
The genetic differentiation in A. stephensi based on haplotype diversity using Restriction Fragment Length Polymorphism and bysequencing of CO II gene across different localities in India has been analyzed. The presence of only one DraI restriction site in CO II gene conferred to haplotype B indicating that the gene is very much conserved and the gene flow is not affected even by a major geographical distance barrier. The sequencing and analysisof various population parameters revealed seven haplotypes in all populations. The West Bengal population was found to be more genetically diverse than others. The geographic distance between populations was found to be contributing to the genetic differentiation. The sign of demographic expansion were found in three of the five populations. The local geographic barriers were found to be ineffective in prevention of gene flow.
基于限制性片段长度多态性的单倍型多样性以及通过对印度不同地区的斯氏按蚊CO II基因进行测序，分析了该物种的遗传分化情况。CO II基因中仅存在一个DraI限制性位点，这赋予了单倍型B，表明该基因高度保守，即使存在主要的地理距离屏障，基因流动也未受影响。对各种群体参数的测序和分析显示，所有群体中共有七种单倍型。发现西孟加拉邦的群体在遗传上比其他群体更加多样化。群体之间的地理距离被发现对遗传分化有影响。在五个群体中的三个群体中发现了种群扩张的迹象。发现当地的地理屏障在阻止基因流动方面无效。
