Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Medical Entomology, Disease Control Unit, Urmia Health Center, Urmia University of Medical Sciences, Urmia, Iran.
BMC Res Notes. 2022 Aug 12;15(1):276. doi: 10.1186/s13104-022-06135-8.
Microbial infections and mosquito-borne diseases such as malaria, with 627 k deaths in 2020, are still major public health challenges.
This study prepared nanoemulsion and nanogel containing Artemisia dracunculus essential oil. ATR-FTIR analysis (Attenuated Total Reflection-Fourier Transform InfraRed) confirmed the successful loading of the essential oil in nanoemulsion and nanogel. LC50 values (Lethal Concentration 50%) of nanogel and nanoemulsion against Anopheles stephensi larvae were obtained as 6.68 (2-19 µg/mL) and 13.53 (7-25 µg/mL). Besides, the growth of Staphylococcus aureus after treatment with 5000 μg/mL nanogel and nanoemulsion was reduced by ~ 70%. However, about 20% growth of Pseudomonas aeruginosa was reduced at this dose. Considering the proper efficacy of the nanogel as a larvicide and proper antibacterial effect against S. aureus, it could be considered for further investigations against other mosquitoes' larvae and gram-positive bacteria.
微生物感染和疟疾等蚊媒疾病仍是重大公共卫生挑战，2020 年有 62.7 万人因此死亡。
本研究制备了含有青蒿精油的纳米乳液和纳米凝胶。ATR-FTIR 分析（衰减全反射-傅里叶变换红外）证实了精油在纳米乳液和纳米凝胶中的成功负载。纳米凝胶和纳米乳液对致倦库蚊幼虫的 LC50 值（半数致死浓度）分别为 6.68（2-19μg/mL）和 13.53（7-25μg/mL）。此外，用 5000μg/mL 的纳米凝胶和纳米乳液处理后，金黄色葡萄球菌的生长减少了约 70%。然而，在该剂量下，铜绿假单胞菌的生长减少了约 20%。考虑到纳米凝胶作为杀幼虫剂的适当功效以及对金黄色葡萄球菌的适当抗菌作用，它可能适合进一步研究针对其他蚊子幼虫和革兰氏阳性菌。
