Wang S W
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Dec;11(6):433-7.
Human chromosome DNA from WBC or fetus chorion samples were digested with Hae III and hybridized with biotinylated Y chromosome specific probe by Southern blotting, and hybridization signals were developed by the ABC (Avidin-biotin-alkaline phosphatase complex) system. The hybridization signal for 0.1 microgram of male DNA could be detected clearly, while the signal for even 5 micrograms of female DNA could not. Parallel tests showed that the sexing results using 32P-labeled and biotinylated Y probe were identical. This suggests that the biotinylated Y probe can be applied to the determination of X-linked genetic diseases and sex abnormality, forensic analysis, sex determination of sportsmen and women, heterosexual transplantation of bone marrow, etc. It could become a convenient means for genetic diagnosis.
从白细胞或胎儿绒毛膜样本中提取的人类染色体DNA用Hae III酶消化，通过Southern印迹法与生物素化的Y染色体特异性探针杂交，并用ABC（抗生物素蛋白-生物素-碱性磷酸酶复合物）系统显影杂交信号。0.1微克男性DNA的杂交信号可清晰检测到，而即使5微克女性DNA的信号也无法检测到。平行试验表明，使用32P标记和生物素化Y探针的性别鉴定结果相同。这表明生物素化Y探针可应用于X连锁遗传病和性异常的诊断、法医分析、运动员性别鉴定、骨髓异性移植等。它可能成为一种方便的基因诊断手段。
