Prinz M, Berghaus G, Staak M
Beitr Gerichtl Med. 1989;47:175-9.
DNA was extracted from 1 microliter bloodstains on glass, cotton cloth and paper using proteinase K digestion in a solution containing low gelling temperature agarose. This method is advantageous for small amounts of source material because the purifying procedures of other methods that contain a risk of loss of DNA, are avoided. The recovered DNA was digested with Hae III and after Southern blotting, a Digoxigenin labeled probe (Amprobe, human Y-chromosome specific repeat) was successfully used for sex determination. With this non-radioactive method quantities of DNA smaller than 1.5 pg were detected.
使用蛋白酶K在含有低熔点琼脂糖的溶液中消化，从玻璃、棉布和纸张上的1微升血迹中提取DNA。该方法对于少量样本材料具有优势，因为避免了其他方法中存在DNA损失风险的纯化步骤。回收的DNA用Hae III消化，经过Southern印迹后，成功使用地高辛标记探针（Amprobe，人类Y染色体特异性重复序列）进行性别鉴定。通过这种非放射性方法检测到了小于1.5 pg的DNA量。
