Ohshima T, Haas H, Prinz M, Staak M, Berghaus G
Institut für Rechtsmedizin, Universität zu Köln.
Arch Kriminol. 1990 May-Jun;185(5-6):163-71.
Species- and sex-determination on hair roots were simultaneously performed using extracted DNA and a human Y chromosomespecific probe. (pH Y 2.1) After Southern hybridization, Hae III-digested DNA fragments were detected by non-radioactive digoxigenin detection system. DNA was extracted from one to five freshly plucked hair roots. The specific 2.12 kb fragment was successfully detected in male DNA samples from a single hair root. A positive identification of female DNA was more difficult. The hair root DNA was revealed to be stable at room temperature for at least 2 weeks (examination time) and produced the same specific band pattern as the DNA of fresh hair roots. In the blind tests with DNA samples from randomly plucked one to four hair roots, the rate of successful sex-determination was 95.8% on male samples (23 out of 24 samples) and 25% on female samples (4 out of 16 samples).
使用提取的DNA和人类Y染色体特异性探针（pH Y 2.1）同时对发根进行物种和性别鉴定。Southern杂交后，通过非放射性地高辛检测系统检测Hae III消化的DNA片段。从一到五根新鲜拔下的发根中提取DNA。在来自单个发根的男性DNA样本中成功检测到了特定的2.12 kb片段。对女性DNA进行阳性鉴定则更为困难。结果显示，发根DNA在室温下至少2周（检测时间）内保持稳定，并且产生与新鲜发根DNA相同的特定条带模式。在对随机拔取的一到四根发根的DNA样本进行的盲测中，男性样本的性别鉴定成功率为95.8%（24个样本中的23个），女性样本的成功率为25%（16个样本中的4个）。
