Brutman A V, Ostanin K V, Miasnikov A N, Smirnov M N
Mol Gen Mikrobiol Virusol. 1991 Dec(12):14-9.
Two Saccharomyces cerevisiae strains containing integrated copies of the MAT alpha 1 gene fused to the PHO5 promoter have been obtained by transformation of a MAT alpha 1 mutant. The strains differ in length of 5'-uncoding region of the MAT alpha 1 in the integrated constructions. The mating activity and the ability of these strains to express the yeast MF alpha 1 gene and the hyman alpha-N-interferon gene under the control of MF alpha 1 promoter was shown to be regulated by the exogenous inorganic phosphate. The level of intracellular alpha-N-interferon synthesized in these strains was several fold higher as compared with the wild type alpha mating type strain. At the same time the observed increase in intracellular production is not accompanied by an increase in the level of secreted alpha-N-interferon. On the contrary, one of the strains had a two-fold reduction in the rate of secretion.
通过对 MATα1 突变体进行转化，获得了两个含有与 PHO5 启动子融合的 MATα1 基因整合拷贝的酿酒酵母菌株。这些菌株在整合构建体中 MATα1 的 5' 非编码区长度上有所不同。结果表明，这些菌株的交配活性以及在 MFα1 启动子控制下表达酵母 MFα1 基因和人α-N-干扰素基因的能力受外源无机磷酸盐的调节。与野生型α交配型菌株相比，这些菌株中合成的细胞内α-N-干扰素水平高出几倍。同时，观察到的细胞内产量增加并未伴随着分泌的α-N-干扰素水平的增加。相反，其中一个菌株的分泌速率降低了两倍。
