Aiyetan Paul, Zhang Bai, Chen Lily, Zhang Zhen, Zhang Hui
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, United States of America.
J Bioinform. 2014 Apr 28;1(2):40-49.
Proteome Discoverer is one of many tools used for protein database search and peptide to spectrum assignment in mass spectrometry-based proteomics. However, the inadequacy of conversion tools makes it challenging to compare and integrate its results to those of other analytical tools. Here we present M2Lite, an open-source, light-weight, easily pluggable and fast conversion tool. M2Lite converts proteome discoverer derived MSF files to the proteomics community defined standard - the mzIdentML file format. M2Lite's source code is available as open-source at https://bitbucket.org/paiyetan/m2lite/src and its compiled binaries and documentation can be freely downloaded at https://bitbucket.org/paiyetan/m2lite/downloads.
Proteome Discoverer是基于质谱的蛋白质组学中用于蛋白质数据库搜索和肽段到质谱峰匹配的众多工具之一。然而，转换工具的不足使得将其结果与其他分析工具的结果进行比较和整合具有挑战性。在此，我们展示了M2Lite，这是一个开源、轻量级、易于插入且快速的转换工具。M2Lite将Proteome Discoverer生成的MSF文件转换为蛋白质组学界定义的标准——mzIdentML文件格式。M2Lite的源代码可在https://bitbucket.org/paiyetan/m2lite/src上作为开源获取，其编译后的二进制文件和文档可在https://bitbucket.org/paiyetan/m2lite/downloads上免费下载。
