Retief D H, Wendt S L, Bradley E L, Denys F R
Am J Dent. 1989 Oct;2(5):269-73.
The objective was to determine the effect of storage media and the duration of storage of extracted teeth on the shear bond strength (SBS) of Scotchbond 2/Silux to dentin. Extracted human mandibular and maxillary first permanent molars were stored in buffered formalin (A), 1% chloramine (B), 70% ethanol (C), physiological saline (D), or 0.05% thymol (E). Fifteen test specimens were prepared on teeth stored in each medium two days after extraction (Procedures A1 to E1) or after six months storage (Procedures A2 to E2). The teeth were embedded in brass tooth cups and the occlusal surfaces ground wet on 180 and then 600 grit SiC immediately prior to the preparation of a test specimen. The Scotchprep Primer and the Scotchbond 2 Adhesive were applied to the dentin surfaces as directed by the manufacturer. Cylinders of Silux Composite were bonded to the treated dentin surfaces in a special device. The specimens were removed 15 minutes after cure and stored in distilled water at 37 degrees C for 24 hours. A shear load was applied to the bonded cylinders in an Instron machine at a crosshead speed of 0.02 inch.min-1. The SBS were calculated and expressed in MN.m-2. The data were analyzed by ANOVA and Duncan's multiple range test at the 5% level of significance.2+e mean SBS + SD
目的是确定储存介质和拔牙储存时间对Scotchbond 2/Silux与牙本质之间剪切粘结强度（SBS）的影响。拔除的人类下颌和上颌第一恒磨牙分别储存在缓冲甲醛（A）、1%氯胺（B）、70%乙醇（C）、生理盐水（D）或0.05%百里酚（E）中。在拔牙后两天（步骤A1至E1）或储存六个月后（步骤A2至E2），从每种储存介质中的牙齿制备15个测试样本。将牙齿嵌入黄铜牙杯中，在制备测试样本前，立即用180目然后600目的碳化硅砂纸湿磨咬合面。按照制造商的指示，将Scotchprep底漆和Scotchbond 2粘合剂应用于牙本质表面。在一个特殊装置中，将Silux复合材料圆柱体粘结到处理过的牙本质表面。固化15分钟后取出样本，在37℃的蒸馏水中储存24小时。在Instron机器上以0.02英寸·分钟-1的十字头速度对粘结的圆柱体施加剪切载荷。计算SBS并以MN.m-2表示。数据采用方差分析和Duncan多重极差检验，显著性水平为5%。2+e平均SBS+标准差
