Strydom C, Retief D H, Russell C M, Denys F R
Department of Restorative Dentistry, Faculty of Dentistry, University of Stellenbosch, Republic of South Africa.
Am J Dent. 1995 Apr;8(2):93-8.
To determine in vitro (1) the shear bond strengths (SBS) of the modified Gluma system to enamel and dentin; (2) the qualitative and the quantitative microleakage (ML) of Class V preparations on the cemento-enamel junction (CEJ) and in dentin, respectively, restored with the Gluma system; and (3) the effect of the system's components on enamel and dentin by scanning electron microscopy.
Seventy-five extracted human maxillary permanent central incisors and 75 permanent first and second molars were embedded in brass tooth cups with cold-cure acrylic resin. The facial surfaces of the anterior and the occlusal surfaces of the molar teeth were ground wet on 180- followed by 600-grit silicon carbide paper. Demarcated sites on the surfaces were treated according to the manufacturer's instructions and Pekafill composite was bonded to the treated surfaces. Fifteen specimens prepared on enamel were removed 1 minute after cure (A) and the SBS determined. The remaining specimens were removed 15 minutes after final cure, stored in saline at 37 degrees C for 24 hours (B), for 1 week without (C) and with temperature cycling (D), and for 4 weeks (E). Similar regimens were used for dentin, groups F, G, H, I and J. The SBS were determined and expressed in MPa. The data were analyzed by ANOVA, Student-Newman-Keuls and t-tests. For the qualitative ML evaluation, Class V cavities were prepared on the CEJ of 30 human premolars and restored with Gluma/Pekafill. After thermocycling in 0.5% basic fuchsin, the teeth were sectioned and ranked according to the degree of microleakage. Class V cavities were prepared on the facial root surfaces of 15 human premolars and restored. The ML was determined quantitatively by a spectrophotometric dye-recovery method and expressed as microgram dye/restoration.
The mean +/- SD of the SBS in MPa were: A: 14.6 +/- 2.2; B: 21.2 +/- 1.6; C: 20.5 +/- 1.4; D: 24.2 +/- 2.0; E: 22.8 +/- 3.7; F: 7.0 +/- 2.0; G: 16.1 +/- 4.0; H: 15.6 +/- 3.7; I: 10.9 +/- 3.3; J: 14.4 +/- 3.3. The quantitative ML was 1.00 +/- 0.39 microgram dye/restoration. The lowest SBS were obtained on specimens tested after 1 minute. Temperature cycling had a significantly adverse effect on the SBS to dentin (P = 0.0003). Etch patterns conducive to bonding were produced on the enamel. The resin system penetrated into the dentin tubules.
体外测定（1）改良型Gluma系统与釉质和牙本质的剪切粘结强度（SBS）；（2）分别用Gluma系统修复的位于牙骨质 - 釉质界（CEJ）和牙本质中的V类洞型的定性和定量微渗漏（ML）；（3）通过扫描电子显微镜观察该系统各成分对釉质和牙本质的影响。
将75颗拔除的人上颌恒中切牙和75颗恒第一、第二磨牙用冷固化丙烯酸树脂包埋于黄铜牙托中。前牙的唇面和磨牙的咬合面先用180目接着用600目碳化硅砂纸湿磨。根据制造商的说明处理表面上划定的部位，然后将Pekafill复合树脂粘结到处理过的表面。在固化1分钟后（A组）取下15个制备于釉质上的标本并测定SBS。其余标本在最终固化15分钟后取下，于37℃的盐水中储存24小时（B组）、储存1周（无温度循环，C组；有温度循环，D组）以及储存4周（E组）。牙本质标本采用类似方案，分为F、G、H、I和J组。测定SBS并以MPa表示。数据采用方差分析、Student - Newman - Keuls检验和t检验进行分析。对于定性ML评估，在30颗人前磨牙的CEJ处制备V类洞并用Gluma/Pekafill修复。在0.5%碱性品红中进行热循环后，将牙齿切片并根据微渗漏程度分级。在15颗人前磨牙的牙根唇面制备V类洞并修复。通过分光光度法染料回收法定量测定ML并以微克染料/修复体表示。
以MPa为单位的SBS平均值±标准差为：A组：14.6±2.2；B组：21.2±1.6；C组：20.5±1.4；D组：24.2±2.0；E组：22.8±3.7；F组：7.0±2.0；G组：16.1±4.0；H组：15.6±3.7；I组：10.9±3.3；J组：14.4±3.3。定量ML为1.00±0.39微克染料/修复体。在1分钟后测试的标本上获得最低的SBS。温度循环对与牙本质的SBS有显著不利影响（P = 0.0003）。在釉质上产生了有利于粘结的蚀刻图案。树脂系统渗入牙本质小管。
