一种从β发夹文库中鉴定出拟肽基序的催化剂选择方案。
A catalyst selection protocol that identifies biomimetic motifs from β-hairpin libraries.
机构信息
Department of Chemistry, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
出版信息
J Am Chem Soc. 2014 Nov 12;136(45):15817-20. doi: 10.1021/ja503012g. Epub 2014 Nov 4.
PMID:25347708
Abstract
Assaying a solid-phase library of histidine-containing β-hairpin peptides by a reactive tagging scheme in organic solvents selects for catalysts that reproduce the strategies used by His-based enzyme active sites to accelerate acyl- and phosphonyl-transfer reactions. Rate accelerations (k(rel)) in organic solvents of up to 2.4 × 10(8) are observed.
摘要
通过在有机溶剂中使用反应性标记方案对含有组氨酸的β发夹肽固相文库进行分析，可选择出能够重现基于组氨酸的酶活性部位用于加速酰基和膦酰基转移反应的策略的催化剂。在有机溶剂中观察到高达 2.4×10(8)的速率加速(k(rel))。
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