The role of β-hairpin conformation in ester hydrolysis peptide catalysts based on a TrpZip scaffold.

To explore the role of peptide conformation on catalytic activity in the context of ester hydrolysis catalysts, pairs of sequences were designed that contained or lacked β-hairpin character. For the hydrolysis of -nitrophenylacetate in aqueous media, we found small but consistent trends wherein His-containing sequences based on a scaffold showed higher catalytic activity without β-hairpin character.