Prévéraud D P, Devillard E, Rouffineau F, Borel P
Adisseo France S.A.S., Center of Expertise and Research in Nutrition, 6 route Noire, 03600 Commentry, France INSERM, UMR1062, "Nutrition, Obesity and Risk of Thrombosis," 13385 Marseille, France INRA, UMR1260, 13385 Marseille, France Aix-Marseille University, Faculté de Médecine, 13385 Marseille, France
Adisseo France S.A.S., Center of Expertise and Research in Nutrition, 6 route Noire, 03600 Commentry, France.
J Anim Sci. 2014 Nov;92(11):4972-80. doi: 10.2527/jas.2013-7099.
A study was performed in growing pigs to evaluate the efficacy of α-tocopherol (Tol) concentration in plasma, muscle, liver, and adipose tissue following dietary supplementation with vitamin E (VE) and various sources of fat. The trial involved 96 piglets weaned at an average of 28 d of age. Piglets were fed for 2 wk a semipurified diet not supplemented with VE. Piglets were then randomly assigned to 5 isoenergetic semipurified diets with 100 IU/kg VE as dl-α-tocopheryl acetate: a control (CTRL) diet (with no added fat) and 4 other diets containing either 3% linseed oil (LIN), 3% hydrogenated coconut oil (COC), 3% olive oil (OLI), or 3% safflower oil (SAF) representing diets rich in n-3 PUFA, SFA, MUFA, and n-6 PUFA, respectively. After 49 d of treatment, pigs were killed and blood, muscle (longissimus dorsi), adipose tissue, and whole liver (without gallbladder) were collected and analyzed for their Tol concentrations. For all tissues, LIN and SAF diets led to lower (P < 0.02) Tol concentrations as compared to the CTRL diet: -63 and -67%, respectively. α-Tocopherol concentrations in plasma, liver, and adipose tissue were greater (P < 0.001) in the COC group as compared to the CTRL group. The OLI diet led to greater (P < 0.01) liver Tol concentration (+92%) as compared to the CTRL diet but had no significant effect on plasma, muscle, and adipose tissue Tol concentrations. There were significant correlations (P < 0.001) between plasma, muscle, and liver Tol concentrations (r > 0.78). These results show that supplementation with PUFA markedly decreases Tol concentration in blood and tissues of growing pigs, whereas SFA increase Tol content in blood, liver, and adipose tissue. Monounsaturated fatty acids only increase liver Tol concentrations. Therefore, increasing the amount of fat in the diet (from <0.1 to approximately 3.5%) and the type of dietary fatty acids supplemented with VE are key factors with regards to VE concentration in plasma and tissue. The Tol:PUFA needs to be carefully considered to meet the VE pigs requirement and to ensure an optimal Tol meat enrichment.
进行了一项针对生长猪的研究，以评估日粮补充维生素E（VE）和各种脂肪来源后，血浆、肌肉、肝脏和脂肪组织中α-生育酚（Tol）的浓度。该试验涉及96头平均28日龄断奶的仔猪。仔猪先饲喂2周不添加VE的半纯化日粮。然后将仔猪随机分为5种等能量的半纯化日粮，日粮中添加100 IU/kg的dl-α-生育酚乙酸酯：一种对照（CTRL）日粮（不添加脂肪）和4种其他日粮，分别含有3%的亚麻籽油（LIN）、3%的氢化椰子油（COC）、3%的橄榄油（OLI）或3%的红花油（SAF），分别代表富含n-3多不饱和脂肪酸（PUFA）、饱和脂肪酸（SFA）、单不饱和脂肪酸（MUFA）和n-6 PUFA的日粮。处理49天后，处死猪，采集血液、肌肉（背最长肌）、脂肪组织和整个肝脏（不带胆囊），并分析其Tol浓度。对于所有组织，与CTRL日粮相比，LIN和SAF日粮导致Tol浓度降低（P<0.02）：分别降低了63%和67%。与CTRL组相比，COC组血浆、肝脏和脂肪组织中的α-生育酚浓度更高（P<0.001）。与CTRL日粮相比，OLI日粮使肝脏Tol浓度更高（P<0.01）（增加了92%），但对血浆、肌肉和脂肪组织中的Tol浓度没有显著影响。血浆、肌肉和肝脏中的Tol浓度之间存在显著相关性（P<0.001）（r>0.78）。这些结果表明，补充PUFA会显著降低生长猪血液和组织中的Tol浓度，而SFA会增加血液、肝脏和脂肪组织中的Tol含量。单不饱和脂肪酸仅会增加肝脏中的Tol浓度。因此，日粮中脂肪含量的增加（从<0.1%增加到约3.5%）以及补充VE的日粮脂肪酸类型是影响血浆和组织中VE浓度的关键因素。需要仔细考虑Tol:PUFA的比例，以满足猪对VE的需求并确保肉中Tol的最佳富集。
