Evavold B D, Yokoyama A, Hirsch R, Bluestone J A, Quintans J
Committee on Immunology, University of Chicago, IL 60637.
Int Immunol. 1989;1(4):443-9. doi: 10.1093/intimm/1.4.443.
We have investigated the effects of monoclonal antibody (mAb) to the CD3 epsilon protein on interactions between small, resting T cells and antigen-specific T helper clones. Highly purified, splenic T cells lacking identifiable accessory cells do not proliferate in a thymidine uptake assay to anti-CD3 mAb, Con A, rIL-2, rIL-4, or irradiated T helper clones (both Th1 and Th2). However, the responding T cells proliferate significantly to the combined stimulus of Th2 clones and anti-CD3 antibody. Only the Th2, not the Th1, subpopulation of T helper cells has the ability to induce a T cell response. The Th2 cell-dependent activation of small resting T cells does not require the external cross-linkage of the anti-CD3 mAb via Fc receptor expressing cells or the secretion of lymphokines from the Th2 helper clones, but it is inhibitable by anti-LFA 1 antibody. Thus, Th2 clones provide a co-stimulatory signal which in conjunction with anti-CD3 mAb causes resting T cell proliferation in the absence of conventional accessory cells.
我们研究了抗CD3ε蛋白单克隆抗体（mAb）对小型静止T细胞与抗原特异性T辅助细胞克隆之间相互作用的影响。在缺乏可识别辅助细胞的情况下，高度纯化的脾T细胞在胸腺嘧啶核苷摄取试验中，对抗CD3 mAb、刀豆蛋白A、重组白细胞介素-2（rIL-2）、重组白细胞介素-4（rIL-4）或经照射的T辅助细胞克隆（Th1和Th2）均无增殖反应。然而，反应性T细胞对Th2克隆和抗CD3抗体的联合刺激有显著增殖。只有T辅助细胞的Th2亚群，而非Th1亚群，具有诱导T细胞反应的能力。小型静止T细胞的Th2细胞依赖性激活不需要通过表达Fc受体的细胞对抗CD3 mAb进行外部交联，也不需要Th2辅助细胞克隆分泌淋巴因子，但可被抗淋巴细胞功能相关抗原-1（LFA-1）抗体抑制。因此，Th2克隆提供了一种共刺激信号，该信号与抗CD3 mAb共同作用，可在无传统辅助细胞的情况下使静止T细胞增殖。
