Günther T, Vormann J
Institute of Molecular Biology and Biochemistry, Free University of Berlin, Fed. Rep. of Germany.
J Trace Elem Electrolytes Health Dis. 1989 Jun;3(2):89-92.
Pregnant Wistar rats were treated with 5 oral doses of 100 or 300 mg/kg salicylic acid from day 16 to 20 of gestation. At day 21 of gestation, copper concentrations were measured in serum, liver and cell organelles of liver from dams and litters after cell fractionation. Salicylate caused a reduction of maternal serum Cu and an increase in fetal liver Cu. The increase of Cu in fetal liver was proportional to the Cu content of the organelles. It was concluded that salicylate bound Cu and transferred Cu from maternal serum to the fetus. The increase in fetal liver Cu was explained by displacement of Zn from Zn-metallothionein by Cu, because Zn-metallothionein is high in fetal liver and Cu has a higher affinity to metallothionein than Zn. Such a mechanism was not significant in maternal liver because of the low Zn-metallothionein content in maternal liver.
妊娠第16至20天，给妊娠的Wistar大鼠口服5次剂量为100或300mg/kg的水杨酸。在妊娠第21天，对母鼠及其幼崽进行细胞分级分离后，测定血清、肝脏以及肝脏细胞器中的铜浓度。水杨酸盐导致母鼠血清铜含量降低，胎儿肝脏铜含量升高。胎儿肝脏中铜的增加与细胞器中的铜含量成正比。得出的结论是，水杨酸盐与铜结合，并将铜从母鼠血清转移至胎儿。胎儿肝脏中铜的增加是由于铜取代了锌金属硫蛋白中的锌，因为胎儿肝脏中锌金属硫蛋白含量较高，且铜对金属硫蛋白的亲和力高于锌。由于母鼠肝脏中锌金属硫蛋白含量较低，这种机制在母鼠肝脏中并不显著。
