Elliott R C, Pedersen T J, Fristensky B, White M J, Dickey L F, Thompson W F
Department of Botany, North Carolina State University, Raleigh 27695.
Plant Cell. 1989 Jul;1(7):681-90. doi: 10.1105/tpc.1.7.681.
We have isolated, mapped, and sequenced a genomic clone containing the ferredoxin I (Fed-1) gene from Pisum sativum. The gene is present as a single copy per haploid genome. It has no introns, and it specifies a 753-nucleotide transcript encoding a 149-amino acid protein including a 52-residue transit peptide. Upstream sequences from Fed-1 contain several elements with similarity to transcriptional regulatory elements from RbcS and Cab genes, and gel mobility shift assays show that nuclear extracts from light-grown pea leaves contain one or more DNA binding activities specific for Fed-1 5'-flanking sequences. RbcS and Cab regulatory sequences are only weak competitors for this binding, however, and the RbcS and Cab similarities mostly lie outside of the region essential for binding. These data are discussed in terms of previously observed physiological differences between the light responses of Fed-1 and other genes.
我们已经从豌豆中分离、定位并测序了一个包含铁氧化还原蛋白I（Fed-1）基因的基因组克隆。该基因在单倍体基因组中以单拷贝形式存在。它没有内含子，其转录本为753个核苷酸，编码一个149个氨基酸的蛋白质，其中包括一个52个残基的转运肽。Fed-1的上游序列包含几个与RbcS和Cab基因的转录调控元件相似的元件，凝胶迁移率变动分析表明，光生长豌豆叶片的核提取物含有一种或多种对Fed-1 5'侧翼序列具有特异性的DNA结合活性。然而，RbcS和Cab调控序列对这种结合的竞争较弱，而且RbcS和Cab的相似性大多位于结合所必需区域之外。结合之前观察到的Fed-1与其他基因光反应之间的生理差异对这些数据进行了讨论。
