McKnight S L, Kingsbury R
Science. 1982 Jul 23;217(4557):316-24. doi: 10.1126/science.6283634.
Transcriptional control signals of a model eukaryotic protein-coding gene have been identified by a new procedure of in vitro mutagenesis. This method allows small clusters of nucleotide residues to be substituted in a site-directed manner without causing the addition or deletion of other sequences. Transcription assays of a systematic series of these clustered point mutants have led to the identification of three distinct control signals located within the 105-nucleotide residues immediately upstream from the point where transcription begins.
一种新型的体外诱变程序已鉴定出一个典型真核生物蛋白质编码基因的转录控制信号。该方法能够以定点方式替换一小簇核苷酸残基，而不会导致其他序列的添加或缺失。对这些成簇点突变体的一系列系统转录分析，已鉴定出位于转录起始点上游紧邻的105个核苷酸残基内的三种不同控制信号。
