Leisner S M, Gelvin S B
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Plant Cell. 1989 Sep;1(9):925-36. doi: 10.1105/tpc.1.9.925.
It is known that a 16-base pair palindrome (ACGTAAGCGCTTACGT) located upstream of the ocs gene can activate a maize adh1 promoter in a transient expression system [Ellis et al. (1987). EMBO J. 6, 11-16; Ellis et al. (1987). EMBO J. 6, 3203-3208]. We have determined that this palindrome is also essential for ocs promoter activity in tobacco calli. In addition, sequences immediately adjacent to this palindrome, both 5' and 3', modulate its activity. The palindrome is sensitive to the differentiated state of the plant cells in which it resides; it is active in calli and the leaves of small shoots but is inactive in the leaves of rooted plants. We have tested upstream sequences from two other T-DNA genes that have homology to this palindrome for their ability to activate the octopine synthase promoter in tobacco calli. The upstream region from the mannopine synthase gene can activate the octopine synthase promoter, but an upstream region from the gene implicated in octopine and nopaline secretion cannot activate the promoter.
已知ocs基因上游的一个16个碱基对的回文序列（ACGTAAGCGCTTACGT）在瞬时表达系统中可激活玉米adh1启动子[埃利斯等人（1987年）。《欧洲分子生物学组织杂志》6，11 - 16；埃利斯等人（1987年）。《欧洲分子生物学组织杂志》6，3203 - 3208]。我们已经确定这个回文序列对于烟草愈伤组织中ocs启动子的活性也是必不可少的。此外，紧邻这个回文序列的5'和3'端序列会调节其活性。该回文序列对其所在植物细胞的分化状态敏感；它在愈伤组织和小芽的叶片中具有活性，但在生根植物的叶片中无活性。我们已经测试了另外两个与该回文序列具有同源性的T - DNA基因的上游序列在烟草愈伤组织中激活章鱼碱合酶启动子的能力。甘露碱合酶基因的上游区域可以激活章鱼碱合酶启动子，但与章鱼碱和胭脂碱分泌相关的基因的上游区域不能激活该启动子。
