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使用Ti质粒载体将嵌合基因导入烟草后，该基因的光诱导性及叶绿体相关表达

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector.

作者信息

Herrera-Estrella L, Van den Broeck G, Maenhaut R, Van Montagu M, Schell J, Timko M, Cashmore A

出版信息

Nature. 1984;310(5973):115-20. doi: 10.1038/310115a0.

DOI:10.1038/310115a0
PMID:6330570
Abstract

A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

摘要

一个嵌合基因已被导入烟草植株的基因组中，该嵌合基因由豌豆编码核酮糖1,5 - 二磷酸羧化酶的基因家族成员的5'侧翼区与细菌氯霉素乙酰转移酶基因的编码区连接而成，使用的是根癌农杆菌的Ti质粒。在含有叶绿体的转化组织中，该嵌合基因的表达受光诱导。

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