利用凝集素微阵列分析建立结直肠癌远处复发的新预测标志物。
Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis.
作者信息
Nakajima Kentaro, Inomata Masafumi, Iha Hidekatsu, Hiratsuka Takahiro, Etoh Tsuyoshi, Shiraishi Norio, Kashima Kenji, Kitano Seigo
机构信息
Department of Gastrointestinal and Pediatric Surgery, Oita University Faculty of Medicine, Hasama-machi, Oita, Japan.
出版信息
Cancer Med. 2015 Feb;4(2):293-302. doi: 10.1002/cam4.342. Epub 2014 Oct 30.
We evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis of cell surface glycan modifications. Glycoproteins were extracted from formalin-fixed, paraffin-embedded tumor specimens and normal epithelium from 53 consecutive curatively resected stage I-III colorectal cancer cases and then subjected to lectin microarray to obtain lectin-glycan interaction (LGI) values. In addition, clinicopathological factors associated with distant recurrence were identified. LGI values that were associated with distant recurrence were validated with an additional 55 curatively resected stage II colorectal cancer cases. LGI values for Agaricus bisporus (ABA) lectin, prominent in cancer tissues, were statistically associated with distant recurrence. ABA lectin staining exhibited strikingly intense signals in the cytoplasm and apical surfaces of cancer cells, while weak staining was observed in the supranuclear regions of normal epithelium. This ABA tumor/normal LGI ratio may be a new predictive biomarker for distant recurrence of curatively resected colorectal cancer.
我们使用细胞表面聚糖修饰的凝集素微阵列分析，评估了用于预测结直肠癌远处复发的新型预测标志物的临床益处。从53例连续接受根治性切除的I - III期结直肠癌病例的福尔马林固定、石蜡包埋肿瘤标本及正常上皮中提取糖蛋白，然后进行凝集素微阵列分析以获得凝集素 - 聚糖相互作用（LGI）值。此外，还确定了与远处复发相关的临床病理因素。用另外55例接受根治性切除的II期结直肠癌病例验证了与远处复发相关的LGI值。双孢蘑菇（ABA）凝集素的LGI值在癌组织中显著，与远处复发具有统计学相关性。ABA凝集素染色在癌细胞的细胞质和顶端表面呈现出强烈的信号，而在正常上皮的核上区域观察到较弱的染色。这种ABA肿瘤/正常LGI比值可能是根治性切除结直肠癌远处复发的一种新的预测生物标志物。
Zotero 插件