Department of Toxicological and Bromatological Sciences (DCTB), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.
Linking Landscape, Environment, Agriculture and Food (LEAF), Higher Institute of Agronomy, Universidade de Lisboa, 1349-017y Lisboa, Portugal.
Int J Mol Sci. 2019 Jun 22;20(12):3059. doi: 10.3390/ijms20123059.
Experiments conducted in vitro and in vivo, as well as some preclinical trials for cancer therapeutics, support the antineoplastic properties of lectins. A screening of antitumoral activity on HT29 colon cancer cells, based on polypeptide characterization and specific lectin binding to HT29 cells membrane receptors, was performed in order to assess the bioactivities present in four Mediterranean plant species: subsp. , subsp. , and . Total leaf proteins from each species were evaluated with respect to cell viability and inhibitory activities on HT29 cells (cell migration, matrix metalloproteinase -MMP proteolytic activities). A discussion is presented on a possible mechanism justifying the specific binding of lectins to HT29 cell receptors. All species revealed the presence of proteins with affinity to HT29 cell glycosylated receptors, possibly explaining the differential antitumor activity exhibited by the two most promising species, subsp. and .
在体和体外实验以及一些癌症治疗的临床前试验都支持凝集素的抗肿瘤特性。为了评估四种地中海植物物种（、和）中存在的生物活性，基于多肽特性和特定凝集素与 HT29 细胞膜受体的结合，对 HT29 结肠癌细胞的抗肿瘤活性进行了筛选。从每种物种中提取总叶蛋白，以评估其对 HT29 细胞（细胞迁移、基质金属蛋白酶-MMP 蛋白水解活性）的细胞活力和抑制活性。本文讨论了一种可能的机制，该机制可以解释凝集素与 HT29 细胞受体的特异性结合。所有物种都显示出与 HT29 细胞糖基化受体具有亲和力的蛋白质的存在，这可能解释了两种最有前途的物种（和）表现出的差异抗肿瘤活性。
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