Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Belgrade, Serbia.
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Methods Mol Biol. 2022;2460:207-222. doi: 10.1007/978-1-0716-2148-6_13.
Lectin-based protein microarrays are used for glycoprofiling of various kinds of biological samples. Here we describe lectin-based microarray assay in the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied the described method for glycan analysis of glycoprotein biomarkers of colorectal cancer associated with the insulin-like growth factor system.
基于凝集素的蛋白质微阵列用于各种生物样品的糖组学分析。在这里，我们描述了基于凝集素的反向微阵列分析方法，其中糖蛋白样品点样于微阵列玻片上，然后与一组凝集素孵育。这种配置允许通过一组凝集素对大量样本进行高通量筛选，而无需将聚糖从糖蛋白中分离出来。我们应用所描述的方法分析与胰岛素样生长因子系统相关的结直肠癌的糖蛋白生物标志物的聚糖。
