Patheja Pooja, Dasgupta Raktim, Dube Alok, Ahlawat Sunita, Verma Ravi Shanker, Gupta Pradeep Kumar
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013, India.
Department of Theory and Bio-systems, Max Planck Institute of Colloids and Interfaces, 14476, Potsdam, Germany.
J Biophotonics. 2015 Sep;8(9):694-704. doi: 10.1002/jbio.201400039. Epub 2014 Oct 29.
The use of optical trap and microbeam for investigating mechanical and transport properties of inter cellular tunneling nanotubes (TnTs) in tumor spheroids has been demonstrated. TnTs in tumor spheroids have been visualized by manipulating TnT connected cells using optical tweezers. Functionality of the TnTs for transferring cytoplasmic vesicles and injected dye molecules by optoporation method has been studied. Further, the TnTs could be longitudinally stretched by manipulating the connected cells and their elastic response was studied. Manipulation of cells at the surface of tumor spheroid using optical tweezers and injection of fluorescent dye into a trapped cell using optoporation technique.
已证明使用光镊和微束来研究肿瘤球体中细胞间隧道纳米管（TnTs）的力学和传输特性。通过使用光镊操纵与TnT相连的细胞，已观察到肿瘤球体中的TnTs。已研究了TnTs通过光穿孔法转移细胞质囊泡和注射染料分子的功能。此外，通过操纵相连的细胞可使TnTs纵向拉伸，并对其弹性响应进行了研究。使用光镊操纵肿瘤球体表面的细胞，并使用光穿孔技术将荧光染料注入捕获的细胞中。
