Carvalho Otávio V, Saraiva Giuliana L, Ferreira Caroline G T, Felix Daniele M, Fietto Juliana L R, Bressan Gustavo C, Almeida Márcia R, Silva Júnior Abelardo
Department of Preventive Veterinary Medicine, Virus Section (Carvalho, Saraiva, Ferreira, Silva Júnior) and Department of Biochemistry and Molecular Biology (Felix, Fietto, Bressan, Almeida), Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, Minais Gerais, Brazil.
Can J Vet Res. 2014 Oct;78(4):283-9.
Canine distemper is a highly contagious disease with high incidence and lethality in the canine population. The objective of this study was to evaluate the efficacy of antiviral action with ribavirin (RBV), interferon-alpha (IFNα), and combinations of RBV and IFNα against canine distemper virus (CDV). Vero cells inoculated with CDV were treated with RBV, IFNα, and combinations of these drugs. The efficacy to inhibit viral replication was evaluated by adding the compounds at different times to determine which step of the viral replicative process was affected. Both drugs were effective against CDV in vitro. The IFNα was the most active compound, with an average IC50 (50% inhibitory concentration) value lower than the IC50 of the RBV. Ribavirin (RBV) was more selective than IFNα, however, and neither drug showed extracellular antiviral activity. The combination of RBV and IFNα exhibited antiviral activity for the intra- and extracellular stages of the replicative cycle of CDV, although the intracellular viral inhibition was higher. Both RBV and IFNα showed high antiviral efficacy against CDV, and furthermore, RBV + IFNα combinations have shown greater interference range in viral infectivity. These compounds could potentially be used to treat clinical disease associated with CDV infection.
犬瘟热是一种在犬类群体中具有高发病率和致死率的高度传染性疾病。本研究的目的是评估利巴韦林（RBV）、α干扰素（IFNα）以及RBV与IFNα联合使用对犬瘟热病毒（CDV）的抗病毒作用效果。用RBV、IFNα以及这些药物的组合处理接种了CDV的Vero细胞。通过在不同时间添加化合物来评估抑制病毒复制的效果，以确定病毒复制过程的哪个步骤受到影响。两种药物在体外对CDV均有效。IFNα是最具活性的化合物，其平均IC50（50%抑制浓度）值低于RBV的IC50。然而，利巴韦林（RBV）比IFNα更具选择性，且两种药物均未表现出细胞外抗病毒活性。RBV与IFNα的组合对CDV复制周期的细胞内和细胞外阶段均表现出抗病毒活性，尽管细胞内病毒抑制作用更高。RBV和IFNα对CDV均显示出高抗病毒效果，此外，RBV + IFNα组合在病毒感染性方面表现出更大的干扰范围。这些化合物有可能用于治疗与CDV感染相关的临床疾病。
