Department of Veterinary Medical Science, Alma Mater Studiorum, University of Bologna, Italy.
Antiviral Res. 2011 Dec;92(3):447-52. doi: 10.1016/j.antiviral.2011.10.004. Epub 2011 Oct 13.
Canine distemper virus (CDV) is a contagious and multisystemic viral disease that affects domestic and wild canines as well as other terrestrial and aquatic carnivores. The disease in dogs is often fatal and no specific antiviral therapy is currently available. In this study, we evaluated the in vitro antiviral activity against CDV of proanthocyanidin A2 (PA2), a phenolic dimer belonging to the class of condensed tannins present in plants. Our results showed that PA2 exerted in vitro antiviral activity against CDV with a higher selectivity index compared to ribavirin, included in our study for the previously tested anti-CDV activity. The time of addition assay led us to observe that PA2 was able to decrease the viral RNA synthesis and to reduce progeny virus liberation, at different times post infection suggesting multiple mechanisms of action including inhibition of viral replicative complex and modulation of the redox milieu. These data suggest that PA2, isolated from the bark of Aesculus hippocastanum, has potential usefulness as an anti-CDV compound inhibiting viral replication.
犬瘟热病毒（CDV）是一种传染性的、全身性的病毒疾病，可感染家养和野生犬科动物以及其他陆地和水生肉食动物。犬科动物的这种疾病通常是致命的，目前尚无特效的抗病毒治疗方法。在这项研究中，我们评估了原花青素 A2（PA2）对 CDV 的体外抗病毒活性，PA2 是一种属于植物中缩合单宁类的二酚类化合物。我们的研究结果表明，PA2 对 CDV 具有体外抗病毒活性，其选择性指数高于利巴韦林，利巴韦林是我们研究中用于检测抗 CDV 活性的药物。添加时间测定实验使我们观察到，PA2 能够降低病毒 RNA 的合成，并减少感染后不同时间的病毒释放，这表明其具有多种作用机制，包括抑制病毒复制复合物和调节氧化还原环境。这些数据表明，PA2 作为一种抑制病毒复制的抗 CDV 化合物，从欧洲七叶树的树皮中分离出来具有潜在的用途。
