Laboratório de Virologia Animal (LVA), Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Res Vet Sci. 2013 Oct;95(2):717-24. doi: 10.1016/j.rvsc.2013.04.013. Epub 2013 May 8.
Infection caused by canine distemper virus (CDV) is a highly contagious disease with high incidence and lethality in the canine population. Antiviral activity of flavonoids quercetin, morin, rutin and hesperidin, and phenolic cinnamic, trans-cinnamic and ferulic acids were evaluated in vitro against the CDV using the time of addition assay to determine which step of the viral replicative cycle was affected. All flavonoids displayed great viral inhibition when they were added at the times 0 (adsorption) and 1h (penetration) of the viral replicative cycle. Both quercetin and hesperidin presented antiviral activity at the time 2h (intracellular). In the other hand, cinnamic acid showed antiviral activity at the times 0 and 2h while trans-cinnamic acid showed antiviral effect at the times -1h (pre-treatment) and 0 h. Ferulic acid inhibited CDV replicative cycle at the times 0 and 1h. Our study revealed promising candidates to be considered in the treatment of CDV. Structural differences among compounds and correlation to their antiviral activity were also explored. Our analysis suggest that these compounds could be useful in order to design new antiviral drugs against CDV as well as other viruses of great meaning in veterinary medicine.
犬瘟热病毒（CDV）感染是一种具有高度传染性的疾病，在犬群中的发病率和致死率都很高。使用添加时间测定法评估了槲皮素、桑色素、芦丁和橙皮苷这 4 种类黄酮以及肉桂酸、反式肉桂酸和阿魏酸这 3 种酚酸对 CDV 的抗病毒活性，以确定病毒复制周期的哪个步骤受到影响。当这些类黄酮在病毒复制周期的 0 小时（吸附）和 1 小时（渗透）时添加时，所有类黄酮都显示出很强的病毒抑制作用。槲皮素和橙皮苷在 2 小时（细胞内）时具有抗病毒活性。另一方面，肉桂酸在 0 小时和 2 小时时具有抗病毒活性，而反式肉桂酸在 -1 小时（预处理）和 0 小时时具有抗病毒作用。阿魏酸在 0 小时和 1 小时时抑制 CDV 复制周期。我们的研究揭示了有希望的候选药物，可以考虑用于治疗 CDV。还探索了化合物之间的结构差异及其与抗病毒活性的相关性。我们的分析表明，这些化合物可用于设计针对 CDV 以及兽医医学中其他具有重要意义的病毒的新型抗病毒药物。
