Al-Amrah Hadba Jar-Allah, Aboznada Osama Abdullah, Alam Mohammad Zubair, ElAssouli M-Zaki Mustafa, Mujallid Mohammad Ibrahim, ElAssouli Sufian Mohamad
Biological Sciences Department, Faculty of Science, King Abdulaziz University , Jeddah , Saudi Arabia and.
Inhal Toxicol. 2014 Dec;26(14):891-6. doi: 10.3109/08958378.2014.970787. Epub 2014 Oct 30.
Waterpipe smoke causes DNA damage in peripheral blood leukocytes and in buccal cells of smokers.
To determine the exposure effect of waterpipe smoke on buccal cells and peripheral blood leukocytes in regard to DNA damage using comet assay.
The waterpipe smoke condensates were analyzed by gas chromatography-mass spectrometry (GC-MS). The study was performed on 20 waterpipe smokers. To perform comet assay on bucaal cells of smokers, 10 µl of cell suspension was mixed with 85 µl of pre-warmed 1% low melting agarose, applied to comet slide and electrophoresed. To analyze the effect of smoke condensate in vitro, 1 ml of peripheral blood was mixed with 10 µl of smoke condensate and subjected for comet assay.
The GC-MS analysis revealed the presence of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4on, nicotine, hydroxymethyl furancarboxaldehyde and 3-ethoxy-4-hydroxybenzaldehyde in the smoke condensates. Waterpipe smoking caused DNA damage in vivo in buccal cells of smokers. The tail moment and tail length in buccal cells of smokers were 186 ± 26 and 456 ± 71, respectively, which are higher than control. The jurak and moassel smoke condensates were found to cause DNA damage in peripheral blood leukocytes. The moassel smoke condensate was more damaging.
There is wide misconception that waterpipe smoking is not as harmful as cigarette smoking. This study demonstrated that waterpipe smoke induced DNA damage in exposed cells.
Waterpipe smokes cause DNA damage in buccal cells. The smoke condensate of both jurak and moassel caused comet formation suggesting DNA damage in peripheral blood leukocytes.
水烟烟雾会导致吸烟者外周血白细胞和口腔细胞中的DNA损伤。
使用彗星试验确定水烟烟雾对口腔细胞和外周血白细胞DNA损伤的暴露效应。
通过气相色谱-质谱联用仪（GC-MS）分析水烟烟雾冷凝物。对20名水烟吸烟者进行了研究。为了对吸烟者的口腔细胞进行彗星试验，将10微升细胞悬液与85微升预热的1%低熔点琼脂糖混合，涂覆在彗星载玻片上并进行电泳。为了分析烟雾冷凝物在体外的作用，将1毫升外周血与10微升烟雾冷凝物混合并进行彗星试验。
GC-MS分析显示烟雾冷凝物中存在2,3-二氢-3,5-二羟基-6-甲基-4H-吡喃-4-酮、尼古丁、羟甲基糠醛和3-乙氧基-4-羟基苯甲醛。水烟吸食在体内导致吸烟者口腔细胞中的DNA损伤。吸烟者口腔细胞中的尾矩和尾长分别为186±26和456±71，高于对照组。发现朱拉克和穆塞尔烟雾冷凝物会导致外周血白细胞中的DNA损伤。穆塞尔烟雾冷凝物的损伤更大。
人们普遍存在一种误解，认为水烟吸食不像香烟吸食那样有害。这项研究表明水烟烟雾会在暴露的细胞中诱导DNA损伤。
水烟烟雾会导致口腔细胞中的DNA损伤。朱拉克和穆塞尔的烟雾冷凝物都导致了彗星形成，表明外周血白细胞中的DNA损伤。
