DehghanNezhad Mehdi, Jalayer Naderi Noushin, Semyari Hassan
Faculty of Dentistry, Shahed University, Tehran, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University.
Iran J Pathol. 2020 Spring;15(2):75-80. doi: 10.30699/ijp.2020.101701.2010. Epub 2020 Feb 26.
BACKGROUND & OBJECTIVE: Micronucleus assay of buccal mucosa cells is a simple bio- monitoring method for diagnosing the genetic damages of toxic agents. The aim was to study the genotoxic effect of waterpipe smoking on buccal mucosa cells using micronucleus assay.
This was a case control. A total of 30 male waterpipe smokers and 30 non-smokers were included in the study. The exfoliated buccal mucosa cells were scrapped using wooden spatula and were spread over glass slides. The mean number of micronuclei was determined using Feulgen-stained slides. The number of micronuclei per 1000 cells was calculated and compared between the two groups of smokers and non-smokers.
The mean number of micronuclei in waterpipe smokers and non-smokers was 1.94±0.39 and 1.680.35, respectively. The micronuclei count in waterpipe smokers was significantly higher than non-smokers (=0). The difference between the number of waterpipe smoking and micronuclei count was significantly different (=0).
The mean number of micronuclei in buccal mucosa cells of waterpipe smokers was significantly higher than non-smokers. The genotoxicity effect of waterpipe was dose-dependent.
颊黏膜细胞微核试验是一种用于诊断有毒物质遗传损伤的简单生物监测方法。本研究旨在通过微核试验研究水烟吸食对颊黏膜细胞的遗传毒性作用。
本研究为病例对照研究。共纳入30名男性水烟吸食者和30名非吸烟者。用木质刮勺刮取脱落的颊黏膜细胞，铺展在载玻片上。使用Feulgen染色的玻片确定微核的平均数量。计算每1000个细胞中的微核数量，并在两组吸烟者和非吸烟者之间进行比较。
水烟吸食者和非吸烟者的微核平均数量分别为1.94±0.39和1.68±0.35。水烟吸食者的微核计数显著高于非吸烟者（P=0）。水烟吸食次数与微核计数之间的差异具有显著性（P=0）。
水烟吸食者颊黏膜细胞中的微核平均数量显著高于非吸烟者。水烟的遗传毒性作用呈剂量依赖性。
