Li E, Becker A, Stanley S L
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Infect Immun. 1989 Jan;57(1):8-12. doi: 10.1128/iai.57.1.8-12.1989.
To study the relationship between carbohydrate-specific amebic cytoadherence and ameba-mediated cytotoxicity, we measured Entamoeba histolytica trophozoite-mediated cytolysis directed against a panel of four Chinese hamster ovary (CHO) cell lines that have defined alterations in their glycosylation patterns. We recently measured amebic trophozoite adherence to this panel of CHO cells and showed that trophozoites bind variant cells (RICR 15B), which are deficient in Asn-linked N-acetyllactosamine units, at 12% of the level observed for wild-type cells (E. Li, A. Becker, and S. L. Stanley, J. Exp. Med 167:1725-1730, 1988). Using a 51Cr release assay to measure trophozoite-mediated cytolysis, we demonstrate in this study that RICR 15B cells are less susceptible to trophozoite-mediated cytolysis than are wild-type cells. In addition, we found that N-acetyllactosamine, which inhibits trophozoite adherence to CHO cells, also inhibited trophozoite-mediated cytolysis of wild-type cells. These studies indicate that surface carbohydrates on target cells can influence susceptibility to ameba-mediated cytotoxicity. This panel of CHO cells provides a useful model system for investigating the role of glycoconjugates in mediating amebic interactions with mammalian cells.
为了研究碳水化合物特异性阿米巴细胞黏附与阿米巴介导的细胞毒性之间的关系，我们检测了溶组织内阿米巴滋养体对一组四种中国仓鼠卵巢（CHO）细胞系介导的细胞溶解作用，这些细胞系在糖基化模式上有明确的改变。我们最近检测了阿米巴滋养体对这组CHO细胞的黏附情况，结果显示滋养体与缺乏N-连接的N-乙酰乳糖胺单元的变异细胞（RICR 15B）结合，其结合水平仅为野生型细胞的12%（E. Li、A. Becker和S. L. Stanley，《实验医学杂志》167:1725 - 1730，1988年）。在本研究中，我们使用51Cr释放试验来检测滋养体介导的细胞溶解作用，结果表明RICR 15B细胞比野生型细胞对滋养体介导的细胞溶解作用更不敏感。此外，我们发现抑制滋养体与CHO细胞黏附的N-乙酰乳糖胺也能抑制滋养体对野生型细胞的细胞溶解作用。这些研究表明，靶细胞表面的碳水化合物可以影响对阿米巴介导的细胞毒性的易感性。这组CHO细胞为研究糖缀合物在介导阿米巴与哺乳动物细胞相互作用中的作用提供了一个有用的模型系统。
