Veit M, Schmidt M F, Rott R
Institut für Virologie, Justus-Liebig-Universität Giessen, Federal Republic of Germany.
Virology. 1989 Jan;168(1):173-6. doi: 10.1016/0042-6822(89)90417-0.
Different paramyxoviruses were analyzed for the covalent attachment of fatty acids into their structural proteins. The fusion protein (F) of Newcastle diseases virus and the hemagglutinin-neuraminidase (HN) of Simian virus 5 are fatty acylated, whereas the glycoproteins of Sendai virus are fatty acid free. The fatty acid linkage is labile to treatment with hydroxylamine. SDS-PAGE in the presence of mercaptoethanol releases some of the covalently bound acyl chains.
对不同的副粘病毒进行分析，以研究脂肪酸与它们结构蛋白的共价连接情况。新城疫病毒的融合蛋白（F）和猴病毒5的血凝素-神经氨酸酶（HN）都进行了脂肪酸酰化，而仙台病毒的糖蛋白则不含脂肪酸。脂肪酸连接对羟胺处理不稳定。在巯基乙醇存在下进行的SDS-PAGE会释放一些共价结合的酰基链。
