Schlesinger M J, Cahill D
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110.
Virology. 1989 Jan;168(1):187-90. doi: 10.1016/0042-6822(89)90421-2.
Two agents, verapamil and chlorpromazine, which affect calcium levels in cells were tested as inhibitors of Sindbis and vesicular stomatitis virus replication in chicken embryo fibroblasts. At levels which did not greatly inhibit general protein synthesis, both drugs appeared to selectively block virus formation at the final stages of virus assembly and budding. Viral nucleoprotein synthesis and transport of viral glycoproteins to the cell plasma membrane were not inhibited under conditions that blocked virus particle release into the cell culture medium. The effects of the drugs were reversible but no morphologically distinct virus-specific structures were detected in the inhibited cells.
维拉帕米和氯丙嗪这两种影响细胞内钙水平的药物，被作为辛德毕斯病毒和水疱性口炎病毒在鸡胚成纤维细胞中复制的抑制剂进行了测试。在对总体蛋白质合成没有显著抑制的水平下，这两种药物似乎在病毒装配和出芽的最后阶段选择性地阻断了病毒形成。在阻止病毒颗粒释放到细胞培养基中的条件下，病毒核蛋白合成以及病毒糖蛋白向细胞质膜的转运并未受到抑制。药物的作用是可逆的，但在受抑制的细胞中未检测到形态上明显的病毒特异性结构。
