Constantinescu S N, Cernescu C D, Popescu L M
Division of Cell Biology, Faculty of Medicine, Bucharest, Romania.
FEBS Lett. 1991 Nov 4;292(1-2):31-3. doi: 10.1016/0014-5793(91)80826-o.
The protein kinase C inhibitor H-7 (2-20 microM) inhibited dose-dependently the infectivity of the vesicular stomatitis virus on cultured human fibroblasts. Electron microscopy showed that H-7 inhibited the viral entry. H-7 also inhibited the infectivity of four other enveloped viruses, herpes simplex I, turkey herpes, vaccinia and Sindbis. Similar results were obtained using staurosporine (2.5 nM), tamoxifen (40 microM), phloretin (140 microM), or W-7 (40 microM). However, the infectivity of non-enveloped viruses (e.g. poliomyelitis I) was not inhibited by H-7. These results show that protein kinase C is critically involved in the infectivity of enveloped viruses, most probably at the level of viral entry (receptor-mediated endocytosis).
蛋白激酶C抑制剂H-7（2 - 20微摩尔）剂量依赖性地抑制水疱性口炎病毒对培养的人成纤维细胞的感染性。电子显微镜显示H-7抑制病毒进入。H-7还抑制其他四种包膜病毒单纯疱疹I型、火鸡疱疹病毒、痘苗病毒和辛德毕斯病毒的感染性。使用星形孢菌素（2.5纳摩尔）、他莫昔芬（40微摩尔）、根皮素（140微摩尔）或W-7（40微摩尔）可获得类似结果。然而，H-7不抑制非包膜病毒（如脊髓灰质炎I型）的感染性。这些结果表明蛋白激酶C在包膜病毒的感染性中起关键作用，很可能是在病毒进入（受体介导的内吞作用）水平。
