Sato H, Furuno A, Yoshiike K
Department of Enteroviruses, National Institute of Health, Tokyo, Japan.
Virology. 1989 Jan;168(1):195-9. doi: 10.1016/0042-6822(89)90423-6.
Human papillomavirus type 16 (HPV 16) open reading frames (ORF) E6, E7, and E6E7, placed under the control of dexamethasone-inducible mouse mammary tumor virus long terminal repeat, were introduced into rat 3Y1 cells, an immortalized fibroblast line, with the aid of neomycin-selection. The cell clones containing inducible HPV 16 ORFs were selected and examined for DNA synthesis. Following induction of HPV mRNA synthesis by the hormone, DNA synthesis was stimulated in the cells containing E7 or E6E7 ORFs. The data indicate that expression of the HPV 16 E7 gene is mitogenic for rat 3Y1 cells.
将人乳头瘤病毒16型（HPV 16）的开放阅读框（ORF）E6、E7和E6E7，置于地塞米松诱导型小鼠乳腺肿瘤病毒长末端重复序列的控制之下，借助新霉素选择，导入永生的成纤维细胞系大鼠3Y1细胞中。选择含有可诱导HPV 16 ORF的细胞克隆，并检测其DNA合成情况。在用激素诱导HPV mRNA合成后，含有E7或E6E7 ORF的细胞中的DNA合成受到刺激。数据表明，HPV 16 E7基因的表达对大鼠3Y1细胞具有促有丝分裂作用。
