Kiyono T, Nagashima K, Ishibashi M
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Nagoya, Japan.
Virology. 1989 Dec;173(2):551-65. doi: 10.1016/0042-6822(89)90567-9.
A transformed cell line (RC335) showing higher saturation cell density was obtained from 3Y1 cells (a fibroblastic cell line of rat) transfected with DNA of human papillomavirus type 47 (HPV-47), an epidermodysplasia verruciformis-associated virus, which our laboratory reported previously. The cell line was found to produce a major and several minor species of viral RNAs. The primary structure of the major viral RNA in RC335 was extensively studied and found to consist of two exons and contain open reading frames (ORFs) E6, E7, and a fused ORF, E1/E4. The major RNA was indicated to play an important role in the transformation of RC335 by an experiment with the recombinant retrovirus designed to produce the RNA containing these ORFs, i.e., the recombinant virus induced transformation similar to that in RC335 upon infection of 3Y1 cells. Furthermore the experiments with recombinant viruses carrying a nonsense mutation or large deletion in the above ORF(s) indicated that E6 was necessary and sufficient for the transformation.
我们实验室先前报道过，从用疣状表皮发育不良相关病毒47型（HPV - 47）DNA转染的3Y1细胞（大鼠成纤维细胞系）中获得了一种显示出更高饱和细胞密度的转化细胞系（RC335）。发现该细胞系能产生一种主要的和几种次要的病毒RNA种类。对RC335中主要病毒RNA的一级结构进行了广泛研究，发现它由两个外显子组成，并包含开放阅读框（ORF）E6、E7以及一个融合的ORF，即E1/E4。通过用设计用于产生包含这些ORF的RNA的重组逆转录病毒进行实验表明，主要RNA在RC335的转化中起重要作用，也就是说，重组病毒在感染3Y1细胞时诱导的转化与RC335中的转化相似。此外，用在上述ORF中携带无义突变或大片段缺失的重组病毒进行的实验表明，E6对于转化是必要且充分的。
