Zhang Hui, Zheng Junxi, Yi Li, Li Yue, Ma Zhe, Fan Hongjie, Lu Chengping
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P. R. China.
J Microbiol. 2014 Nov;52(11):963-9. doi: 10.1007/s12275-014-4311-x. Epub 2014 Oct 31.
Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type I. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type I binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type I binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis that interact with fibronectin or collagen type I.
猪链球菌是一种主要的猪病原体，是一种新兴的人畜共患病原体，可导致脑膜炎和败血性休克。细菌细胞壁和分泌蛋白通常参与与细胞外基质蛋白（ECM）的相互作用，这在发病机制的初始阶段起着重要作用。在本研究中，二维SDS-PAGE、基于蛋白质印迹的结合亲和力测量和微量滴定板结合试验被用于鉴定猪链球菌中与纤连蛋白和I型胶原蛋白相互作用的细胞壁和分泌蛋白。我们从猪链球菌中鉴定出六种蛋白质，其中三种蛋白质（翻译延伸因子G、寡肽结合蛋白OppA前体和磷酸甘油酸变位酶）同时显示出纤连蛋白和I型胶原蛋白结合活性。据我们所知，这三种新鉴定的蛋白质以前没有报道过纤连蛋白或I型胶原蛋白结合活性。总体而言，本研究的目的是从猪链球菌中鉴定具有ECM结合活性的蛋白质，这是首次报道猪链球菌中六种与纤连蛋白或I型胶原蛋白相互作用的新蛋白质。
